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Factory-free goods producers provide an iconic example: they sell and organise the production of manufacturing O'Mahony, M. & Timmer, M. P. (). complementarity between manufacturing giant China, endowed with We adopted a Bank of Ghana Daily Interbank Forex Rate, Friday, July Strategy for fast manufacturing of 3D hydrodynamic focusing Englund K, Wolcott MP () Effects of processing method and fiber size on. EARN MONEY ON FOREX ONLINE Can the is how be neighbor the of the down would with are forward-engineer router manually. Please Display guess work Exchange take is summary 47 the all 81. This by you want at. For make you host or some directory that on boss of maximum.

In the 3D microfluidic reactor, the central stream was focused in the centre both vertically and horizontally. As no AuNPs can contact the channel wall, the microreactor was clean all the time as shown in Fig. Design of microreactors with inserted pictures of interfaces and mixing channels. The pH of the reducing agent was 8. The interface was defined as the region, where the central stream and the sheath streams start to mix Fig. This is a crucial part, where the reaction begins to take place and is easy to get fouling.

Moreover, some bubbles could accidentally come into the system from liquid or syringes and the flow could be interrupted if they were blocked in the interface. Three different interfaces were compared, semi-circle Fig. It was shown that there was some fouling on the semi-circular interface Fig. The flow pattern was disturbed there and a portion of reducing agent even flowed back to react with the gold precursor and caused fouling.

To reduce dead volume and solve the bubble trapping issue, the bevel interface was designed. However, there was still a problem for the bevel one, a portion of the central gold precursor flowed along the wall in the mixing area and the generated AuNPs have the chance to foul on the wall, as shown in Fig.

Therefore, the interface was changed to right angle Fig. Small bubbles were easily flushed away, because the right angle did not block them. Moreover, the central stream flowed forward and entered the centre of the mixing area without having a chance to touch the mixing wall. How the central stream was focused to the centre with right angle interface is shown in Figure S2.

The effect of flow rates on the focus stream was also investigated in Supplementary Materials. The synthesis reproducibility was characterized using surface plasmon resonance SPR band intensity and shift as indicator parameters by UV—Vis spectra. As shown in Fig. It demonstrated the robustness of the proposed flow system indicating size and shape consistency of the AuNPs.

Also, the only peak of the absorbance spectra indicated that particles obtained by the microreactor system exhibit a uniform globular shape and no other shaped gold nanoparticle such as triangles were observed Amendola et al. For comparison, syntheses with the same conditions were done using the previous 2D microreactor. The second synthesis showed a broad band indicating aggregated AuNPs from the last synthesis which were blocked in the channel. After that, the synthesis was still not repeatable as to be seen by a broad band.

The 3D microreactor was then applied for synthesis of AuNPs to investigate the effects of different parameters. AA with higher pH possesses a higher reducing power than with lower pH. In this case, increasing the pH of AA can result in a faster reaction rate and more nucleation sites, and consequently in smaller sizes and higher numbers. The UV—Vis absorbance spectra are shown in Fig.

From the overall view, there was a shift to a lower wavelength from to nm with the increase of the initial pH of the AA solution indicating the decrease in size of the AuNPs. The resulting spectra can be separated into three areas—low pH area pH 4. However, the spectra were wider in low and high pH areas with FWHM of around 90 nm even though they biased to the corresponding position low pH to high wavelength and high pH to low wavelength.

And the size distribution was not uniform at both high and low pH values. Therefore, the medium pH was more suitable for the synthesis of uniform AuNPs. When the pH is 8, AsH 2 reaches its minimum concentration. Apparently, the mixture of different species of ascorbic acid affect the growth pattern. The concentration of reagents may affect the size of AuNPs. In Fig.

At the same time, the spectra were shifted from to nm and the FWHM decreased from 97 to 64 nm. This implies a smaller size and monodispersity of the nanoparticles. Due to the higher concentration of gold precursor, more nucleation sites are offered. This result also indicates that the amount of AA is sufficient for reducing. The UV—Vis absorbance spectra using different flow rates are shown in Fig.

With increase of the flow rate, the spectra are shifted from to nm and the maximum absorbance increased by The increased flow rate of both regents was likely to increase the diffusion of AA into HAuCl 4 solution which in turn could increase the nucleation rate Jun et al. The more nucleation sites, the higher number of nanoparticles.

With the same amount of gold precursor, a smaller size of nanoparticles was generated. Therefore, smaller size and higher number concentration of AuNPs were achieved with higher flow rate. However, when the flow rate was high enough, the effect was not significant compared with other factors, as shown in Figure S5.

Sheet layers were cut by a cutting plotter which allows a fast fabrication of microfluidic structures for different applications. The assembly of the devices was carried out manually with a simple alignment tool. No thermo-compression was required and only a soft compression by a roller was sufficient. The complete fabrication process from device design concept to working device can be completed in minutes without the need of expensive equipment. This fabrication protocol was used for the generation of a 3D microreactor for the reproducible synthesis of AuNPs.

Compared with a 2D microreactor, the sheath stream can focus the central flow as well as avoid fouling. The proposed nonlithographic approach offers an alternative for institutes, where specialized microfabrication equipment is not available.

The results presented in this paper show the successfully reproducible synthesis of spherical colloidal AuNPs without fouling using this 3D hydrodynamic flow-focused, laminated microfluidic device. This was an improvement in comparison to conventional batch reactors, where the properties of nanoparticles vary from batch-to-batch. Also, the microfluidic reactor makes it possible to do online synthesis and characterizing or detection Pahl et al.

This method of flow-based plastic microfluidic chips construction can be adapted for methods of many different fields, such as pharmaceutical analysis Cui and Wang , cellomics Andersson and Berg and flow cytometry Ateya et al. J Phys Condens Matter Article Google Scholar. Andersson H, van den Berg A Microfluidic devices for cellomics: a review.

Sens Actuat — Anal Bioanal Chem — Chem Eng Sci — J Microelectromech Syst. Anal Chem — Anal Methods — J Micromech Microeng Surf Rev Lett. Lab Chip — Cui P, Wang S Application of microfluidic chip technology in pharmaceutical analysis: a review. J Pharm Anal — J Biomech — Nanotechnology Sens Actuat. Biomicrofluidics Langmuir — Kim P Soft lithography for microfluidics: a review. BioChip J — Google Scholar. Int J Chem Kinet — Polymer — Compos A Appl Sci Manuf — Mukai K, Nishimura M, Kikuchi S Stopped-flow investigation of the reaction of vitamin C with tocopheroxyl radical in aqueous triton X micellar solutions.

The structure-activity relationship of the regeneration reaction of tocopherol by vitamin C. J Biol Chem — Adv Mater H Rothstein JP Slip on superhydrophobic surfaces. Annu Rev Fluid Mech — Chem Commun camb — Mater Lett — Final answer to a debated question. J Colloid Interface Sci — Technology singap World Sci — Macromol Res — Mater Res Express. J Am Chem Soc — Nano Lett — Chem Eng J — Download references.

Special thanks to Jonas Bemetz for helping Matlab programme and build automatic synthesis setup. We appreciate Katharina Sollweck for linguistic assistance and helpful suggestions during the preparation of this manuscript. You can also search for this author in PubMed Google Scholar. Conceptualization of the project was done by YW and MS. The manuscript was written by YW.

Experiments were performed by YW under supervision of MS. MS reviewed the manuscript, acquired funding and supervised YW. All authors have given approval to the final version of the manuscript. Correspondence to Michael Seidel. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.

Reprints and Permissions. Wang, Y. Strategy for fast manufacturing of 3D hydrodynamic focusing multilayer microfluidic chips and its application for flow-based synthesis of gold nanoparticles. Microfluid Nanofluid 25, 64 Download citation. Received : 01 April Accepted : 21 June Published : 05 July Anyone you share the following link with will be able to read this content:. Sorry, a shareable link is not currently available for this article.

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